Protoplasts obtained from Candida tropicalis grown on alkanes.
نویسندگان
چکیده
A method for the preparation of protoplasts from Candida tropicalis cultivated on n-tetradecane is described. This essentially consists of replacing the mannitol-sorbitol solution of the classical helicase technique by 1 m magnesium sulfate and lowering the pH to 4.1 during incubation in the presence of helicase. The protoplasts thus prepared behave like intact cells and are capable of consuming oxygen in the presence of n-tetradecane, n-decane, 1-decanol, and glucose.
منابع مشابه
Protoplasts Obtained from Candida tropicalis
A method for the preparation of protoplasts from Candida tropicalis cultivated on n-tetradecane is described. This essentially consists of replacing the mannitolsorbitol solution of the classical helicase technique by 1 M magnesium sulfate and lowering the pH to 4.1 during incubation in the presence of helicase. The protoplasts thus prepared behave like intact cells and are capable of consuming...
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عنوان ژورنال:
- Journal of bacteriology
دوره 100 3 شماره
صفحات -
تاریخ انتشار 1969